posted on 2023-01-05, 08:03authored byZhiguo Liu, Ye Yuan, Lili Wang, Xuan Zhao, Lixin Wang, Lihu Wang, Zhihui Zhao, Xin Zhao, Yuetong Chu, Yaning Gao, Fangyuan Yang, Yulu Wang, Qiong Zhang, Jin Zhao, Mengjun Liu
Adenylate cyclase is the key enzyme solely synthesizing
cAMP which
participates in cell metabolism regulations and functions as an intracellular
second messenger. However, the biological functions of plant ACs have
not been elucidated clearly for their poor conservative sequences
and low detectable cAMP. We performed a systematic study of plant
ACs by using Chinese jujube, whose fruit exhibits the highest cAMP
content among plants. Three novel ACs were identified from Chinese
jujube, and two types of methods including in vitro and in vivo were
used to certificate ZjAC1–3 which can catalyze
the conversion of ATP into cAMP. The biological functions of significant
accelerations of seed germination, root growth, and flowering were
found via overexpression of these AC genes in Arabidopsis, and these functions of ACs were further demonstrated by treating
the AC-overexpressing transgenic lines and wild type Arabidopsis with bithionol and dibutyryl-cAMP. At
last, transcriptome data revealed that the underlying mechanism of
the biological functions of ACs might be regulation of the key genes
involved in the circadian rhythm pathway and the hormone signal transduction
pathway. This research established a foundation for further investigating
plant AC genes and provided strong evidence for cAMP serving as a
signaling molecule in plants.