The synthesis inhibition conferred by Nt_DnaA is independent on the 5′UTR.

(A) Schematic depiction of dnaA expression reporter constructs from Felletti et al. [34]. (B–C) Growth curves (gray, blue, and orange) and OD₆₀₀-normalized eGFP fluorescence (green) of cells harboring the dnaA expression reporter constructs depicted in (A), after shift to C-, N-, or P-limited media. Light hues: Nt_dnaA-eGFP constructs; dark hues: constructs with eGFP alone. eGFP-coding sequences are preceded by 5′ untranslated regions (5′UTRs) either of the artificial 6/13 UTR type (B), or from the lac operon of E. coli (C).

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