The inhibitory effects of PCC0208025 against PD-1 and PD-L1 binding.

In HTRF assay, Tag2-PD-1 (20 nM final), PCC0208025 (0.15, 0.46, 1.37, 4.12, 12.35, 37.04, 111.11, 333.33 and 1000 nM, final) or BMS-936559 (0.002, 0.006, 0.024, 0.10, 0.39, 1.56, 6.25, 25 and 100 nM final), and Tag1-PD-L1 (2 nM final) were designed. After pre-incubation, anti-Tag1-EuK and anti-Tag2-XL665 was added into the assay well. The signals (665 nm/620 nm ratio) were obtained on Tecan M200 PRO. HTRF ratio = (OD665 nm/OD620 nm) × 10⁴.

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