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The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias
journal contribution
posted on 1993-10-25, 00:00 authored by D H Evans, E Chipouras, T Toop, John DonaldJohn DonaldWe examined the effect of Ca2+, Cd2+, or Ni2+ on vascular smooth muscle intracellular proteins involved in contraction, using rings of detergent-permeabilized aortae from the spiny dogfish, Squalus acanthias. Addition of Ca2+ stimulated contraction of the vascular smooth muscle, and permeabilization by treatment with Triton X-100 increased the sensitivity to Ca2+ nearly 5 log units, demonstrating that this protocol left contractile and regulatory proteins intact. Addition of 1 microM calmodulin did not increase the sensitivity of the rings to Ca2+, suggesting that this preparation is not leaky to this regulatory protein. Neither Cd2+ nor Ni2+ stimulated contraction of permeabilized rings demonstrating that the previously-described contractile action of these heavy metals is not mediated by direct stimulation of intracellular proteins, rather by interaction with sarcolemmal proteins.
History
Journal
ToxicologyVolume
83Issue
1-3Pagination
1 - 8Publisher
ElsevierLocation
Shannon, IrelandPublisher DOI
ISSN
0300-483XLanguage
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1993, Elsevier IrelandUsage metrics
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AnimalsAortaCadmiumCalciumIn Vitro TechniquesNickelOctoxynolSharksVascular smooth muscleDetergent-permeabilizedScience & TechnologyLife Sciences & BiomedicinePharmacology & PharmacyToxicologyDEPENDENT PROTEIN-KINASEDOGFISH SHARKCALCIUM SENSITIVITYVENTRAL AORTACALMODULINRATCONTRACTILITYINHIBITIONMECHANISM
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