TgGRA14 is not needed for the internalization of host cytosolic proteins early in infection.

Rivera-Cuevas, Yolanda; Mayoral, Joshua; Di Cristina, Manlio; Lawrence, Anna-Lisa E.; Olafsson, Einar B.; Patel, Romir K.; Thornhill, Dishari; Waldman, Benjamin S.; Ono, Akira; Sexton, Jonathan Z.; Lourido, Sebastian; Weiss, Louis M.; Carruthers, Vern B.

doi:10.1371/journal.ppat.1010138.s003

ppat.1010138.s003.docx (314.28 kB)

TgGRA14 is not needed for the internalization of host cytosolic proteins early in infection.

journal contribution

posted on 2021-12-13, 18:36 authored by Yolanda Rivera-Cuevas, Joshua Mayoral, Manlio Di Cristina, Anna-Lisa E. Lawrence, Einar B. Olafsson, Romir K. Patel, Dishari Thornhill, Benjamin S. Waldman, Akira Ono, Jonathan Z. Sexton, Sebastian Lourido, Louis M. Weiss, Vern B. Carruthers

A. Experimental design for the analysis of the internalization of host cytosolic proteins of TgGRA14-deficient parasites. (1) Parasites were treated with 5 μM LHVS for 24 h prior to infection, (2) inducible mCherry HeLa cells were infected with parasites for 4 h, (3) the parasites were harvested at 4 hpi and analyzed by microscopy. B. Quantification of host cytosolic mCherry uptake at 4 hpi by WT or RΔgra14 type I strains treated with DMSO or LHVS for 24 h. At least 200 parasites were analyzed per blinded sample. Data represents the mean from ≥ 3 biological replicates. Statistical analysis was by Student’s t-test. Only statistical differences are shown.

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