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Surface-Patterned DNA Origami Rulers Reveal Nanoscale Distance Dependency of the Epidermal Growth Factor Receptor Activation
journal contribution
posted on 2024-01-25, 01:08 authored by Ivy Mayer, Tina Karimian, Klavdiya Gordiyenko, Alessandro Angelin, Ravi Kumar, Michael Hirtz, Ralf Mikut, Markus Reischl, Johannes Stegmaier, Lu Zhou, Rui Ma, Gerd Ulrich Nienhaus, Kersten S. Rabe, Peter Lanzerstorfer, Carmen M. Domínguez, Christof M. NiemeyerThe nanoscale arrangement
of ligands can have a major effect on
the activation of membrane receptor proteins and thus cellular communication
mechanisms. Here we report on the technological development and use
of tailored DNA origami-based molecular rulers to fabricate “Multiscale
Origami Structures As Interface for Cells” (MOSAIC), to enable
the systematic investigation of the effect of the nanoscale spacing
of epidermal growth factor (EGF) ligands on the activation of the
EGF receptor (EGFR). MOSAIC-based analyses revealed that EGF distances
of about 30–40 nm led to the highest response in EGFR activation
of adherent MCF7 and Hela cells. Our study emphasizes the significance
of DNA-based platforms for the detailed investigation of the molecular
mechanisms of cellular signaling cascades.
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epidermal growth factorcellular signaling cascadesbased analyses revealedmembrane receptor proteinsbased molecular rulerstailored dna origamibased platformsmolecular mechanismsegf receptortechnological developmentsystematic investigationstudy emphasizesnanoscale spacingnanoscale arrangementhighest responsehela cellsegfr ).detailed investigationcells ”adherent mcf7
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