pone.0262173.s005.docx (15.72 kB)
Summary of fatty acid content of cell culture media used in this study.
journal contribution
posted on 2022-01-20, 18:26 authored by L. Madison Kirk, Charlotte Mae K. Waits, Alexander C. Bashore, Beverly Dosso, Allison K. Meyers, Antonio C. Renaldo, Thomas J. DePalma, Kelli N. Simms, Nathaniel Hauser, Chia-Chi Chuang Key, Charles E. McCall, John S. Parks, Susan Sergeant, Carl D. Langefeld, Aleksander Skardal, Elaheh RahbarFatty acid methyl esters (FAME) were extracted and quantified using GC-FID. Means and standard error of the mean are reported of the mass of fatty acid per 200 ul volume of media. Standard media refers to Williams-E media with FBS and contains significant amounts of ARA and other PUFAs. For the purpose of this study Control and LA media were formulated using lipoprotein deficient fetal bovine serum (FBS) to obtain a tighter control over PUFA content.
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specifically linoleic acidgenetic variants nearfatty acid desaturasediv >< pdimensional hepatic constructdimensional culture modelsde novo lipogenesisfatty acid metabolismsignificantly reduced fads1reproducibly displayed allelemodern western dietincluding lipid esterificationdriving human diseasespecific metabolic activityslowest metabolic conversionhuman pufa metabolismlipid metabolismsubsequent metabolismminor alleleincluding obesityhuman pufahuman populationsfads1 activitycardiovascular disease>) displayedvivo tt rs174537 results supportrelevant genepoorly replicatemechanistic explorationinflammatory metabolitesinflammatory mediatorsinflammatory diseasesindividuals homozygoushepatocytes derivedhaplotype blockenriched mediae .,dietary omegadiet interactionscurrent twocholesterol efflux3d hydrogels
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