posted on 2024-01-23, 14:38authored byJiaming Cai, Ye Tao, Lujuan Xing, Jian Zhang, Zixu Wang, Zihan Zhu, Wangang Zhang
In our previous study, yeast-derived peptide Tyr-Pro-Leu-Pro
(YPLP)
was found to prolong treadmill time and relieve muscle fatigue in
ICR mice. The present study aimed to further investigate the antifatigue
mechanism of YPLP. Three doses of YPLP (10, 25, and 50 mg/kg·d)
were given to exercise mice for 4 weeks. Results showed that YPLP
reduced the oxidative response via the nuclear factor erythroid-2-related
factor 2 (Nrf2) pathway and promoted energy metabolism through the
AMP-activated protein kinase (AMPK) pathway. Label-free proteomics
results showed that 81 differential abundance proteins (DAPs) were
regulated by high-dose YPLP. These DAPs belonged to proteasome, mitochondrial,
and muscle proteins. YPLP was mainly involved in proteasome, aminoacyl-tRNA
biosynthesis, focal adhesion, and MAPK signal pathways to enhance
muscle endurance. Furthermore, real-time quantitative PCR and Western
blotting results proved that YPLP upregulated Psmd14 expression and
downregulated p38 MAPK expression. Overall, this study revealed the
mechanism behind YPLP to alleviate exercise fatigue.