Spatial separation of centromeres from the nuclear lamina.

(A) Whole mount testis preparations were labeled with anti-Lamin Dm0 and a DNA stain. Maximum intensity projections with representative spermatocytes at the indicated stages illustrate that the large majority of Cid-EGFP dots is not intimately associated with the nuclear lamina. In case of the S1/2 spermatocyte with three Cid-EGFP dots (top), optical sections cutting through these dots are presented below the projection. (B) Dot plot presenting the separation distance between Cid-EGFP dots and the nuclear lamina at the indicated stages. The number of the analyzed dots is given in the plot, as well as the mean distance (± s.d.). Number of spermatocytes analyzed = 6 (S1/2), 9 (S3), 4 (S4), and 5 (S5). Scale bars = 1 μm (S1/2) and 3 μm (S3 –S5).

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