Single copy transgene of unc-116(+) is functional.

Marom, Ronit; Zhang, Bo; Washington, Megan E.; Song, I-Wen; Burrage, Lindsay C.; Rossi, Vittoria C.; Berrier, Ava S.; Lindsey, Anika; Lesinski, Jacob; Nonet, Michael L.; Chen, Jian; Baldridge, Dustin; Silverman, Gary A.; Sutton, V. Reid; Rosenfeld, Jill A.; Tran, Alyssa A.; Hicks, M. John; Murdock, David R.; Dai, Hongzheng; Weis, MaryAnn; Jhangiani, Shalini N.; Muzny, Donna M.; Gibbs, Richard A.; Caswell, Richard; Pottinger, Carrie; Cilliers, Deirdre; Stals, Karen; Eyre, David; Krakow, Deborah; Schedl, Tim; Pak, Stephen C.; Lee, Brendan H.

doi:10.1371/journal.pgen.1011005.s010

pgen.1011005.s010.pdf (42.78 kB)

Single copy transgene of unc-116(+) is functional.

journal contribution

posted on 2023-11-07, 18:41 authored by Ronit Marom, Bo Zhang, Megan E. Washington, I-Wen Song, Lindsay C. Burrage, Vittoria C. Rossi, Ava S. Berrier, Anika Lindsey, Jacob Lesinski, Michael L. Nonet, Jian Chen, Dustin Baldridge, Gary A. Silverman, V. Reid Sutton, Jill A. Rosenfeld, Alyssa A. Tran, M. John Hicks, David R. Murdock, Hongzheng Dai, MaryAnn Weis, Shalini N. Jhangiani, Donna M. Muzny, Richard A. Gibbs, Richard Caswell, Carrie Pottinger, Deirdre Cilliers, Karen Stals, David Eyre, Deborah Krakow, Tim Schedl, Stephen C. Pak, Brendan H. Lee

(A) RNA sequencing transcripts per million (TPM) of endogenous unc-116 and unc-116 transgene on chromosome II. Rescue of body length (B) and thrashing speed (C) defects were demonstrated upon introduction of two additional copies of unc-116(+) transgene into unc-116 (del). The difference in length between wild type and unc-116(del) with 2 extra copies of unc-116(+) is less than 2% and while statistically different is not considered biologically significant. Student t-test was used for comparison; ns: not significant, **p<0.001.

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