Single Cell
Analysis of Proteoforms

Su, Pei; Hollas, Michael A. R.; Butun, Fatma Ayaloglu; Kanchustambham, Vijaya Lakshmi; Rubakhin, Stanislav; Ramani, Namrata; Greer, Joseph B.; Early, Bryan P.; Fellers, Ryan T.; Caldwell, Michael A.; Sweedler, Jonathan V.; Kafader, Jared O.; Kelleher, Neil L.

doi:10.1021/acs.jproteome.4c00075.s002

pr4c00075_si_002.pdf (1.85 MB)

Single Cell Analysis of Proteoforms

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posted on 2024-03-18, 14:43 authored by Pei Su, Michael A. R. Hollas, Fatma Ayaloglu Butun, Vijaya Lakshmi Kanchustambham, Stanislav Rubakhin, Namrata Ramani, Joseph B. Greer, Bryan P. Early, Ryan T. Fellers, Michael A. Caldwell, Jonathan V. Sweedler, Jared O. Kafader, Neil L. Kelleher

We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold while accessing protein composition with higher coverage.

Single Cell Analysis of Proteoforms

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