posted on 2021-02-22, 21:44authored byChengzhi Lv, Yao Zhang, Ligen Zou, Juan Sun, Xinjie Song, Jianwei Mao, Yuanfeng Wu
Isothiocyanates (ITCs)
are well-known chemopreventive agents that
have received significant interest across the nutrition and pharmaceutical
industries owing to their anticancer properties, thus it is essential
to increase the conversion of glucosinolate to ITCs by myrosinase
to maximize their health benefits. In this paper, using broccoli seed
meals as a raw material, we comparatively analyzed the outcomes of
two extraction methods: (i) hydrolysis followed by extraction (HFE)
and (ii) simultaneous hydrolysis and extraction (SHE) in terms of
the ITC yield. The results revealed that the SHE method showed a relatively
greater erucin production from broccoli seeds and greater antitumor
and antioxidant activities. A similar phenomenon was found for the
hydrolysates of crude myrosinase and crude glucosinolate separated
from broccoli seeds. However, when the crude glucosinolates were hydrolyzed
by purified broccoli myrosinase, or when pure glucoraphanin was hydrolyzed
by crude myrosinase, no significant effects were noted on the types
and yields of ITCs between the SHE and HFE methods.