Similarity between UGA14 plasmid pSLT-BT-UGA14 and reference S. Typhimurium plasmids pSBLT (ST313) and pSLT-BT (D23580).

Kubicek-Sutherland, Jessica Z.; Xie, Gary; Shakya, Migun; Dighe, Priya K.; Jacobs, Lindsey L.; Daligault, Hajnalka; Davenport, Karen; Stromberg, Loreen R.; Stromberg, Zachary R.; Cheng, Qiuying; Kempaiah, Prakasha; Ong’echa, John Michael; Otieno, Vincent; Raballah, Evans; Anyona, Samuel; Ouma, Collins; Chain, Patrick S. G.; Perkins, Douglas J.; Mukundan, Harshini; McMahon, Benjamin H.; Doggett, Norman A.

doi:10.1371/journal.pntd.0008991.s005

pntd.0008991.s005.pdf (23.13 kB)

Similarity between UGA14 plasmid pSLT-BT-UGA14 and reference S. Typhimurium plasmids pSBLT (ST313) and pSLT-BT (D23580).

journal contribution

posted on 2021-02-01, 18:31 authored by Jessica Z. Kubicek-Sutherland, Gary Xie, Migun Shakya, Priya K. Dighe, Lindsey L. Jacobs, Hajnalka Daligault, Karen Davenport, Loreen R. Stromberg, Zachary R. Stromberg, Qiuying Cheng, Prakasha Kempaiah, John Michael Ong’echa, Vincent Otieno, Evans Raballah, Samuel Anyona, Collins Ouma, Patrick S. G. Chain, Douglas J. Perkins, Harshini Mukundan, Benjamin H. McMahon, Norman A. Doggett

The AMR gene island is circled by dashed line, and the direct repeats in pSBLT are highlighted as yellow. There is a 19 kb inversion occurring in pSLT-BT but not in pSLT-BT-UGA14 (35838–54324 bps). There are two pSBLT genes, IS5075 and repA, that are not present in pSLT-BT-UGA14 at the 5’ and 3’ end of inversion boundaries, respectively. Although this hexameric replicative helicase RepA is missing in the UGA14 genome, IncFII family repA is present in pSLT-BT-UGA14, pSBLT and pSLT-BT. Notably, this 19 kb inversion region is overlapped with prophage regions from 52719–73452 and flanked by IS elements from 3 different backgrounds (pSBLT, pSLT-BT, and pSLT-BT-UGA14), indicating a potential recombination hotspot at this locus.

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