posted on 2023-11-09, 18:06authored byZiyue Wang, Lu Li, Haiyang Yan, Wenliang Li, Yong Pang, Yuan Yuan
Furan is a heat-induced food contaminant, and it causes
damage
to visceral organs, including the testis. To determine the mechanism
of the damage to the testis, a mouse model treated with furan (8 mg/kg
bw/day) and salidroside (SAL, 10/20/40 mg/kg bw/day) was established,
and levels of testicular functional markers and changes of morphology
were investigated in furan-induced mice treated with SAL. The change
in related proteins and genes suggested that SAL restored the furan-mediated
leaky tight junction and triggered the TLR4/MyD88/NF-κB pathway
and NLRP3 inflammasome together with inflammation. To find out the
gut-testis axis, microbiota PICRUSt analysis and correlation analysis
were conducted to investigate the core microbiota and metabolites.
The endoplasmic reticulum stress (ERS)-related key protein levels
and the result of transmission electron microscopy suggested that
SAL inhibited the furan-induced intestinal ERS. The result of TUNEL
and levels of apoptosis-related proteins suggested that furan-induced
intestinal apoptosis was alleviated by SAL. Collectively, SAL inhibited
furan-induced ERS-mediated intestinal apoptosis through modulation
of intestinal flora and metabolites, thus strengthening the gut barrier.
It inhibited LPS from entering the circulatory system and suppressed
the testicular TLR4/MyD88/NF-κB pathway and NLRP3 inflammasome,
which alleviated testicular inflammation.