Version 4 2019-10-02, 22:33Version 4 2019-10-02, 22:33
Version 3 2019-09-23, 14:48Version 3 2019-09-23, 14:48
Version 2 2019-09-23, 14:46Version 2 2019-09-23, 14:46
Version 1 2019-09-01, 22:56Version 1 2019-09-01, 22:56
journal contribution
posted on 2019-10-02, 22:33authored byWilliam PerryWilliam Perry, Jeffrey Spraggins, Jessica R. Sheldon, Caroline M. Grunenwald, David E. Heinrichs, James E. Cassat, Eric P. Skaar, Richard M. Caprioli
Siderophores, iron scavenging small
molecules, are fundamental to bacterial nutrient metal acquisition and enable pathogens
to overcome challenges imposed by nutritional immunity. Multimodal imaging mass
spectrometry allows visualization of host-pathogen iron competition by mapping
siderophores within infected tissue. We have observed
heterogeneous distributions of
Staphylococcus aureus siderophores across infectious foci, challenging the
paradigm that the vertebrate host is a uniformly iron deplete environment to
invading microbes. The attached file includes methodological information in the acquisition of data as well as supplemental data.
Funding
Work was funded by the NIH: National Institute of General Medical Sciences (2P41 GM103391-07 (R.M.C.)), the NIH: National Institute of Allergy and Infectious Diseases (NIAID) (R01AI138581 (E.P.S and J.M.S), R01AI069233 (E.P.S), and R01AI073843 (E.P.S.)). The 15T FT-ICR MS and LA-ICP-MS in the Mass Spectrometry Research Center at Vanderbilt University were acquired through the NIH Shared Instrumentation Grant Programs 1S10OD012359 and 1S10RR026742-01A1, respectively. J.E.C. is supported by NIH: NIAID (1R01AI132560 and 1R01AI132560) and a Career Award for Medical Scientists from the Burroughs Wellcome Fund. D.E.H. is supported by an operating grant from the Canadian Institutes of Health Research.