pone.0299755.s003.pdf (102.65 kB)
SDS-PAGE (CBB staining) of purified recombinant Rs89B1and Rs89B1_ins.
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posted on 2024-02-28, 18:31 authored by Hiroyuki Ohashi, Daisuke Koma, Hayato Yamanaka, Takashi OhmotoLane M represents the marker (ExcellBand All Blue Regular Range Protein Marker, SMBIO Technology INC., Taiwan); lane 1 shows purified Rs89B1 (52.4 kDa), and lane 2 displays purified Rs89B1_ins (52.8 kDa). An arrow indicates the predicted molecular weights of the target proteins.
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gt family 1alter natural substancesraphanus sativus arabidopsis thaliana rs89b1 glycosylates primarilytune enzyme propertiesdiv >< psubstrate recognition siterecognition sitepara substrate affinityenzyme engineeringenzymatic propertiesrs89b1 impactrs89b1 ),ugts ),ugt ’study narrowsstudy demonstratessignificant implicationsprofound understandingloop regionhydroxyl groupglycosyltransferase activityglycosyltransferase 89b1extensive applicationsespecially hydroxybenzoatesconserved regionscatalytic activitybiotechnological applicationsbenzene ringalso contribute
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