S5 Fig -

(A) Alternate STORM single rod examples from Rho-GFP/+ IS-enriched retinas immunolabeled with NbGFP-A647 (magenta), and centrin and STX3 antibodies (combined, yellow), and Rab11a. (B) STORM examples for Rho-GFP-1D4/+ IS-enriched retinas immunolabeled with NbGFP-A647 (magenta), and centrin and STX3 antibodies (combined, yellow), and Rab11a. Rab11a+ clusters identified with Voronoi tessellation are in white. (C, D) STORM examples for Rho-GFP-1D4/+ IS-enriched retinas immunolabeled with NbGFP-A647 (magenta), and centrin and STX3 antibodies (combined, yellow), and either (C) DYNC1H1 or (D) Rootletin antibody. (E) Diagram of the mouse rod IS architecture and the localization pattern of Rho. IS, inner segment; NbGFP-A647, GFP nanobody Alexa 647 conjugate; Rho, rhodopsin; STORM, stochastic optical reconstruction microscopy; STX3, syntaxin 3.

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