S2 Fig -

Sarkar, Murshed H.; Yagi, Ryoji; Endo, Yukihiro; Koyama-Nasu, Ryo; Wang, Yangsong; Hasegawa, Ichita; Ito, Toshihiro; Junttila, Ilkka S.; Zhu, Jinfang; Kimura, Motoko Y.; Nakayama, Toshinori

doi:10.1371/journal.pone.0260204.s002

pone.0260204.s002.pdf (1.63 MB)

S2 Fig -

journal contribution

posted on 2021-11-22, 18:23 authored by Murshed H. Sarkar, Ryoji Yagi, Yukihiro Endo, Ryo Koyama-Nasu, Yangsong Wang, Ichita Hasegawa, Toshihiro Ito, Ilkka S. Junttila, Jinfang Zhu, Motoko Y. Kimura, Toshinori Nakayama

(A) A schematic illustration of the experimental protocol using Tbx21^-/- mice. Naïve CD4 T cells were isolated from Tbx21^-/- (CD45.2), Tbx21^+/+ (CD45.2) and congenic C57BL/6 (CD45.1) mice and cultured under Th2 conditions. Tbx21^-/- Th2 cells were mixed together with CD45.1 Wt Th2 cells, labelled with CFSE and cultured with IL-2 in the presence of 4-OHT for the indicated number of days. (B) The Tbx21 mRNA expression relative to Hprt on Th2 cells after treatment with or without IFNγ. Error bars represent the mean ± SD. Data are representative of three independent experiments. (C) Intracellular staining of IL-4 and IFNγ production of Tbx21^+/+ or Tbx21^–/–CD4 T cells cultured for 5 days under Th1-skewing or Th2-skewing conditions is shown. (D) The MFI of CFSE on Th2 cells after cultivation for the indicated number of days. Data are representative of two independent experiments. (E) A flow cytometry analysis showing the MFI of CFSE dilution in Th1 cells after the cultivation with IFNγ or anti-IFNγ Ab for the indicated days.