Fourier shell correlation (FSC) of the final 3D reconstruction following gold standard refinement using RELION [48,49]. The resolutions corresponding to an FSC of 0.143 are shown for (A) unbound AAV9P31, (C) AAV9P31-Car4 complex and (E) 3-fold block of AAV9P31-Car4. (B), (D), (F) The surface of the maps is colored from red to blue indicating the different local resolutions.