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Representative full blot for total protein and target protein staining.

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posted on 2024-03-01, 18:27 authored by Diego Iacono, Kathleen Hatch, Erin K. Murphy, Jeremy Post, Robert N. Cole, Daniel P. Perl, Regina M. Day

For Western blotting, 10 μg of protein was added per well for each sample. For all eight target proteins that underwent WB testing, we show a representative blot with Ponceau S staining for total protein (left) and subsequent target protein staining (right). The band that was used for quantification is indicated by an arrow with approximate molecular weight. APRT (A), SORBS1 (B), TPM1 (C), TPM2 (D), TPM3 (E), TPM4 (F), PCP4 (G) and NPY (H). Quantification results are shown in Fig 3a.

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