Reduction of brat expression delays pupariation in a regeneration-specific manner (Related to Fig 2).

(A) Pupariation rates in undamaged control (w¹¹¹⁸) (n = 221) and brat¹/+ (n = 110) animals, from three independent experiments. (B) Pupariation rates after disc regeneration for control (w¹¹¹⁸) (n = 384), brat¹/+ (n = 107), brat¹⁹²/+ (n = 131), brat¹⁵⁰/+ (n = 114) and brat¹¹/+ (n = 113) animals. Pupariation rates are from the same experiments as in Fig 2A. (C) Nubbin-positive nuclei were counted at R0 in control regenerating (n = 12) and brat¹/+ regenerating (n = 11) discs. (D) PH3-positive nuclei were counted within the regenerating tissue as marked by Anti-Nubbin co-immunostaining. Total number of nuclei were also counted in the Nubbin expressing region. Ratio of PH3-positive nuclei and total Nubbin-positive nuclei for control (w¹¹¹⁸) and brat¹/+ discs at R0 (n = 16 and 18). (E) EdU incorporation marking DNA synthesis in cells in S phase was quantified by normalizing pixel intensity in the regenerating pouch to pixel intensity in the notum. Control n = 7 discs, brat¹/+ n = 5 discs. *p<0.05. *** p < 0.0005. Error bars represent SEM. Student’s T-test used for statistical analyses. Error bars represent SEM.

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