Rapid and Self-Administrable
Capillary Blood Microsampling
Demonstrates Statistical Equivalence with Standard Venous Collections
in NMR-Based Lipoprotein Analysis
posted on 2024-02-19, 12:33authored byJayden
Lee Roberts, Luke Whiley, Nicola Gray, Melvin Gay, Philipp Nitschke, Reika Masuda, Elaine Holmes, Jeremy K. Nicholson, Julien Wist, Nathan G. Lawler
We investigated plasma and serum blood derivatives from
capillary
blood microsamples (500 μL, MiniCollect tubes) and corresponding
venous blood (10 mL vacutainers). Samples from 20 healthy participants
were analyzed by 1H NMR, and 112 lipoprotein subfraction
parameters; 3 supramolecular phospholipid composite (SPC) parameters
from SPC1, SPC2, and SPC3 subfractions;
2 N-acetyl signals from α-1-acid glycoprotein
(Glyc), GlycA, and GlycB; and 3 calculated parameters, SPC (total),
SPC3/SPC2, and Glyc (total) were assessed. Using
linear regression between capillary and venous collection sites, we
explained that agreement (Adj. R2 ≥
0.8, p < 0.001) was witnessed for 86% of plasma
parameters (103/120) and 88% of serum parameters (106/120), indicating
that capillary lipoprotein, SPC, and Glyc concentrations follow changes
in venous concentrations. These results indicate that capillary blood
microsamples are suitable for sampling in remote areas and for high-frequency
longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs.