posted on 2024-03-12, 22:13authored byManleen Kaur, Sadam H. Bhat, Rajnish Tiwari, Pratibha Kale, Dinesh M. Tripathi, Shiv Kumar Sarin, Savneet Kaur, Neetu Singh
Sepsis is a dysregulated inflammatory response leading
to multiple
organ failure. Current methods of sepsis detection are time-consuming,
involving nonspecific clinical signs, biomarkers, and blood cultures.
Hence, efficient and rapid sepsis detection platforms are of utmost
need for immediate antibiotic treatment. In the current study, a noninvasive
rapid monitoring electrochemical sensing (ECS) platform was developed
for the detection and classification of plasma samples of patients
with liver cirrhosis by measuring the current peak shifts using the
cyclic voltammetry (CV) technique. A total of 61 hospitalized cirrhotic
patients with confirmed (culture-positive) or suspected (culture-negative)
sepsis were enrolled. The presence of bacteria in the plasma was observed
by growth kinetics, and for rapidness, the samples were co-encapsulated
in microscaffolds with carbon nanodots that were sensitive enough
to detect redox changes occurring due to the change in the pH of the
surrounding medium, causing shifts in current peaks in the voltammograms
within 2 h. The percentage area under the curve for confirmed infections
was 94 and that with suspected cases was 87 in comparison to 69 and
71 with PCT, respectively. Furthermore, the charge was measured for
class identification. The charge for LPS-absent bacteria ranged from
−400 to −600 μC, whereas the charge for LPS-containing
bacteria class ranged from −290 to −300 μC. Thus,
the developed cost-effective system was sensitive enough to detect
and identify bacterial sepsis.