ppat.1010138.s005.docx (811.92 kB)
RΔgra14:gra14-HA complementation mutants.
journal contribution
posted on 2021-12-13, 18:36 authored by Yolanda Rivera-Cuevas, Joshua Mayoral, Manlio Di Cristina, Anna-Lisa E. Lawrence, Einar B. Olafsson, Romir K. Patel, Dishari Thornhill, Benjamin S. Waldman, Akira Ono, Jonathan Z. Sexton, Sebastian Lourido, Louis M. Weiss, Vern B. CarruthersA. Schematic representation of the plasmids generated for TgGRA14 complementation. B. PCR to validate the presence of TgGRA14-HA in the RΔgra14GRA14 complementation mutants. C. Immunoblot confirming the presence of TgGRA4 and HA in the RΔgra14GRA14 complementation mutants. D. Representative images showing the presence of HA and GRA14 in the RΔgra14GRA14 complementation mutants. Scale bar is 5 μm.
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master manipulator capablelate domain motifsfusogenic parasitophorous vacuolecontains motifs homologousparasite proteins includinghost cytosolic proteinsdense granule proteinsdeficient parasites revealedhost escrt componentsparasite gains resourcestoxoplasma gondii dependent vlp buddinghost escrt machineryparasite uptakehost cytosolhost cellwt parasitesvesicular buddinggondii dense granulesmediate escrtresiding withinreplicative nicherelease assaypvm ).molecular underpinningsmarked reductionlimiting membranelike particlekey pathwayintracellular subsistenceeffectively siphoningcontaining portionalso showaccesses contents1 virus1 gag
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