posted on 2024-03-05, 18:29authored byRuirui Huang, Vivian F. Irish
Bimolecular fluorescence complementation assay (BiFC) to detect reconstitution of YFP fluorescence. YFP fluorescence shows interaction between RBE and TPR2 (top row), TPR3 (middle row) or TPR4 (bottom row) in the nuclei. Position of nuclei detected by fluorescence of H2B-mCherry. Panels (left to right): YFP; H2B-mCherry; merged. Scale bars 50 um.