ppat.1009891.s003.docx (88.47 kB)
Quantification of confocal microscopy data.
journal contribution
posted on 2021-08-31, 17:32 authored by Bhawna Yadav, Charles A. Specht, Chrono K. Lee, Maria Pokrovskii, Jun R. Huh, Dan R. Littman, Stuart M. LevitzMice were challenged with A. fumigatus conidia as described in Fig 2D. BAL cells were permeabilized and stained for RORγt and the eosinophil marker MBP. A total of 205 cells in 25 microscope fields were analyzed by confocal microscopy for the presence of RORγt and MBP. Data are expressed as mean (± SE) percentage of cells staining for the indicated combination of the two markers.
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sup >-/-mcherry reporter mousemaster transcription factor>- challenged wildutilized murine modelslung eosinophils elicitedcomparing lung eosinophilsdiv >< pacute pulmonary aspergillosiseosinophil rorγt expressionacute modelspulmonary aspergillosiseosinophil ilwestern blottingpositively correlatedlive conidiaimmunocompetent personsformer predominatedflow cytometrycytoplasmic localizationconfocal microscopyatopic individualsadverse consequences
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