Purification of HCMV NEC containing His-UL50 and Myc-UL53 with size-exclusion chromatography.

Chen, Han; Lye, Ming F.; Gorgulla, Christoph; Ficarro, Scott B.; Cuny, Gregory D.; Scott, David A.; Wu, Fan; Rothlauf, Paul W.; Wang, Xiaoou; Fernandez, Rosio; Pesola, Jean M.; Draga, Sorin; Marto, Jarrod A.; Hogle, James M.; Arthanari, Haribabu; Coen, Donald M.

doi:10.1371/journal.ppat.1011781.s001

ppat.1011781.s001.pdf (421.93 kB)

Purification of HCMV NEC containing His-UL50 and Myc-UL53 with size-exclusion chromatography.

journal contribution

posted on 2023-11-17, 18:40 authored by Han Chen, Ming F. Lye, Christoph Gorgulla, Scott B. Ficarro, Gregory D. Cuny, David A. Scott, Fan Wu, Paul W. Rothlauf, Xiaoou Wang, Rosio Fernandez, Jean M. Pesola, Sorin Draga, Jarrod A. Marto, James M. Hogle, Haribabu Arthanari, Donald M. Coen

Top: HCMV His-UL50 and Myc-UL53 were purified individually using affinity chromatography and then chromatographed on a size-exclusion column at a ratio of 1 Myc-UL53 to 2 His-UL50 (green trace). A trace of His-UL50 alone is shown for comparison (blue trace). Bottom: Aliquots of fractions corresponding to the faster eluting peak following chromatography of the protein mixture were resolved using SDS-PAGE and the indicated fractions were pooled for use in HTRF assays.

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