pone.0293011.s003.pdf (1023.21 kB)
Protocol for processing slides for multiplexing.
journal contribution
posted on 2024-01-17, 18:30 authored by Chrystal Chadwick, Magdia De Jesus, Fiona Ginty, Jessica S. MartinezProtocol document from protocol io (dx.doi.org/10.17504/protocols.io.bpwumpew), which outlines slide clearing and antigen retrieval procedures to prepare slides for multiplex imaging.
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utmost concern duestill poorly understoodsignificant human morbidityfollowing pathologist assessmentdrug resistant strainsdiv >< pmacrophages ), b220multiplex immunofluorescent imagingcd3 +/ cd45candida auris mxif analysis highlightedinfection mice modelsfungal organisms contributeeither 1a8 —infection within heartsingle cell analysiscells ), cd68cell analysisauris monocytes ),multiplexed imaging8c5 —infected miceinfection analyzedc albicans mechanistic understandingleast abundantincreased countimmune cellshospital settingsheterogenous distributionfungal abscessesfindings suggestdeeper understandingcritical careconcentrated aroundbroaden understandingb cells8c5 antibodies
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