posted on 2024-01-11, 16:18authored byQizhao Tan, Feng Li, Ke Zhang, Zhongjun Liu, Yun Tian, Tengjiao Zhu
Osteoarthritis (OA)
is a prevalent debilitating whole-joint disorder.
Currently, a growing number of proteomic studies have been performed
to evaluate molecular biomarkers in several tissues from OA patients;
however, little is known about the protein profiles in subchondral
bone of OA. In this study, proteomic analysis was performed on subchondral
bone from patients with OA to identify differentially expressed proteins
(DEPs). Bioinformatics tools were used to further investigate these
DEPs. Thereafter, DEPs were validated in the samples from patients
with OA, as well as in bilateral ovariectomy-induced OA (OVX-OA) rats
using immunohistochemistry. A comprehensive subchondral bone proteome
profile of patients with OA was constructed. Additionally, biological
information analysis showed that a majority of DEPs participated in
the dysregulation of the complement and coagulation cascades. The
validation experiments suggested that SerpinA5, the protein involved
in the complement and coagulation cascades, was significantly increased
in severely damaged subchondral bone of patients with OA compared
to the control group. Furthermore, the increase of SerpinA5 in OVX-OA
rats compared to control rats was also confirmed. Our results indicated
that the dysregulation of coagulation and complement pathways plays
a role in the progression of OA, and it provides a promising therapeutic
target of OA.