ppat.1011831.s009.doc (111 kB)
Primers used in the construction of the knock-out, epitope-tagged, complemented and mutant strains.
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posted on 2023-12-13, 18:35 authored by Qin-Li Liang, Lan-Bi Nie, Hany M. Elsheikha, Ting-Ting Li, Li-Xiu Sun, Zhi-Wei Zhang, Meng Wang, Bao-Quan Fu, Xing-Quan Zhu, Jin-Lei WangPrimers used in the construction of the knock-out, epitope-tagged, complemented and mutant strains.
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six conserved motifsphosphoproteomic analysis revealedfunctional studies showeddiv >< pcell cycle progressiontgpp6c significantly reducedtgpp6c significantly attenuatedtgpp6c predominately alteredparasite lytic cycleparasite cell cycletoxoplasma gondii cell divisiontoxoplasma gondii tgpp6c contributesparasite ’parasite growthtranslational regulatorstaken togethersynchronous divisionresults showpys tachyzoitespru cystsproteins involvedprotein phosphatasesphosphorylation statuslow homologylethal dosedisordered rosettesdeficient typedaughter cellscomplex whereasbradyzoite differentiation
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