Primers for RT-qPCR, promoter cloning, full length CDS cloning of TaAAP2, TaAAP13, and TaAAP21 into yeast vector, RNAi and overexpression plasmid vector constructs.

Wan, Yongfang; Wang, Yan; Shi, Zhiqiang; Rentsch, Doris; Ward, Jane L.; Hassall, Kirsty; Sparks, Caroline A.; Huttly, Alison K.; Buchner, Peter; Powers, Stephen; Shewry, Peter R.; Hawkesford, Malcolm J.

doi:10.1371/journal.pone.0246763.s011

pone.0246763.s011.docx (13.86 kB)

Primers for RT-qPCR, promoter cloning, full length CDS cloning of TaAAP2, TaAAP13, and TaAAP21 into yeast vector, RNAi and overexpression plasmid vector constructs.

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posted on 2021-02-19, 18:38 authored by Yongfang Wan, Yan Wang, Zhiqiang Shi, Doris Rentsch, Jane L. Ward, Kirsty Hassall, Caroline A. Sparks, Alison K. Huttly, Peter Buchner, Stephen Powers, Peter R. Shewry, Malcolm J. Hawkesford

Underlined sequences were added restriction enzyme digestion sites.

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Primers for RT-qPCR, promoter cloning, full length CDS cloning of TaAAP2, TaAAP13, and TaAAP21 into yeast vector, RNAi and overexpression plasmid vector constructs.

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