pone.0258891.s004.docx (19.23 kB)

Primer sequences and annealing temperature used in PCR amplification of each gene target.

journal contribution

posted on 2021-10-20, 17:32 authored by Marjo V. Mendoza, Windell L. Rivera

Annealing temperatures are based on optimized PCR conditions for each gene target except for glmU, which was not amplified in all samples. ^aPrimers used for Leptospira detection. ^bMLST schemes 2 and 3 primers published in Leptospira PubMLST database (http://pubmlst.org/leptospira/).

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targeting 23s rrna reduced scheme addressed phylogenetic analysis confirmed one sample cannot method often used loci genetic profile clinically diagnosed cases challenges often encountered allelic profiles identified 16s rrna (<disease epidemiology specifically multilocus sequence typing div >< p simplified mlst scheme human leptospirosis cases rapid direct typing philippines rapid typing direct typing disease surveillance molecular typing rrs2 mrea lipl41 lipl32 leptospira l interrogans adk two serovars tertiary hospital serovar manilae serological tests serogroup pyrogenes routinely done public health potential alternative positive samples pathogen transmission nine positives new st monitor occurrence mlst schemes major threat interpretable data host specificity hospital settings could lead clinical specimens clinical samples clearly differentiated blood samples also aid

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