ppat.1011585.s006.pdf (307.68 kB)
Preparation of PV extracted from iRBC and staining with anti-RBC IgG.
journal contribution
posted on 2023-11-08, 18:50 authored by Padmapriya Sekar, Sumati Rajagopalan, Estela Shabani, Usheer Kanjee, Marc A. Schureck, Gunjan Arora, Mary E. Peterson, Boubacar Traore, Peter D. Crompton, Manoj T. Duraisingh, Sanjay A. Desai, Eric O. Long(A) RBC infected with 3D7 PfEXP2-GFP were stained with membrane dye PKH26 and eFluor 670 and treated with 0.0125% saponin for 3 minutes, followed by a wash and mechanical disruption of the iRBC plasma membrane through a 31-gauge needle. (B) Samples were sorted by flow cytometry for PKH26-negative eFluor 670+ particles. (C) Mean fluorescence intensity (MFI) of uninfected RBC (uninfected RBC), iRBC, and PV stained with different concentrations of anti-RBC IgG. The first set of points represent samples stained with secondary Ab only (sec only).
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within parasitophorous vacuolessup >+releases parasitophorous vacuolesquantitative flow cytometrymechanism may mitigatelytic effector moleculesinducible erythrocyte precursorsfree vesicles similarelectron microscopy imagesapplied gene ablationpeople chronically exposeddiv >< pcells lyse virusmonocytes may combinedependent cellular cytotoxicityplasmodium falciparum nk cells lysenk cells bindadaptive nk cellsrbc plasma membranep falciparum nk cellstransformed cellstarget cellsinfected cellsexposed individualspv membraneselective nknk celltwo orderssubsequent phagocytosisspecific recognitionreleased intactreduced parasitemiaprimary monocytespolarized deliverynatural killermediated adccmagnitude higherlive imagingintegrin αmβ2high frequencyefficient killingcentral rolecell sortingblocking experimentsadcc ).
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