posted on 2021-02-18, 17:35authored byAnne-Isaline van Bohemen, Nicolas Ruiz, Aurore Zalouk-Vergnoux, Aurore Michaud, Thibaut Robiou du Pont, Irina Druzhinina, Lea Atanasova, Soizic Prado, Bernard Bodo, Laurence Meslet-Cladiere, Bastien Cochereau, Franck Bastide, Corentin Maslard, Muriel Marchi, Thomas Guillemette, Yves François Pouchus
In the course of investigations on
peptaibol chemodiversity from
marine-derived Trichoderma spp., five new 15-residue
peptaibols named pentadecaibins I–V (1–5) were isolated from the solid culture of the strain Trichoderma sp. MMS1255 belonging to the T. harzianum species complex. Phylogenetic analyses allowed precise positioning
of the strain close to T. lentiforme lineage inside
the Harzianum clade. Peptaibol sequences were elucidated
on the basis of their MS/MS fragmentation and extensive 2D NMR experiments.
Amino acid configurations were determined by Marfey’s analyses.
The pentadecaibins are based on the sequences Ac-Aib1-Gly2-Ala3-Leu4-Aib/Iva5-Gln6-Aib/Iva7-Val/Leu8-Aib9-Ala10-Aib11-Aib12-Aib13-Gln14-Pheol15. Characteristic of the pentadecaibin
sequences is the lack of the Aib-Pro motif commonly present in peptaibols
produced by Trichoderma spp. Genome sequencing of Trichoderma sp. MMS1255 allowed the detection of a 15-module
NRPS-encoding gene closely associated with pentadecaibin biosynthesis.
Pentadecaibins were assessed for their potential antiproliferative
and antimicrobial activities.