pone.0256701.s001.docx (17.21 kB)
Parameter settings CASA IVOS (Version 12.0 IVOS hamilton thorne bioscience, Beverly, USA).
journal contribution
posted on 2021-09-02, 17:31 authored by Juan Carlos Gutiérrez-Añez, Heiko Henning, Andrea Lucas-Hahn, Ulrich Baulain, Patrick Aldag, Birgit Sieg, Vivian Hensel, Doris Herrmann, Heiner Niemann(DOCX)
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sup >&# 8722plain tyrode ’many biological systemsflow cytometry dataaverage motility parameters>- derived blastocyststhaw sperm qualitysperm subpopulation structureassisted sperm analysisacrosome intact spermembryo development comparedearly embryo development180 min incubationshowed higher (<enhanced embryonic development11 thawed bovine spermatozoamelatonin improves ratediv >< pembryo developmentenhanced (<30 min120 minvitro reduced (<p mapk13 greater (<viable spermrelative abundancepresent studypreparation protocolpotent nonoxidative stressmatured cumulusmainly dueincubated eitherhierarchical clusterfrequently reducedfertilizing abilityfemale gametesenzymatic antioxidantcontrol groupcontrol ).control ),complexes fertilizedadding melatoninactive mitochondria
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