pntd.0009361.s002.docx (29.27 kB)
PCR mix optimized for our Study.
journal contribution
posted on 2021-06-01, 17:41 authored by Odilon Nouatin, Juliana Boex Mengue, Jean Claude Dejon-Agobé, Rolf Fendel, Javier Ibáñez, Ulysse Ateba Ngoa, Jean Ronald Edoa, Bayodé Roméo Adégbité, Yabo Josiane Honkpéhédji, Jeannot Fréjus Zinsou, Aurore Bouyoukou Hounkpatin, Kabirou Moutairou, Andreas Homoet, Meral Esen, Andrea Kreidenweiss, Stephen L. Hoffman, Michael Theisen, Adrian J. F. Luty, Bertrand Lell, Selidji Todagbe Agnandji, Ghyslain Mombo-Ngoma, Michael Ramharter, Peter Kremsner, Benjamin Mordmüller, Ayôla Akim AdegnikaDifferent primers and probes concentrations were tested to evaluate the best one for our assay. We used the following primers and probes concentration for each species: 0.1 μM, 0.2 μM, 0.3 μM and 0.4 μM. After checking and analyzing the PCR amplification curves, we decided to use the 0.2 μM concentration. Table 2 shows the chosen setting for our assay. # Volume depending of the reagent start concentration.
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CHMITotal vaccine-specific IgG concentr...Vaccine-specific antibody concentra...35 dayshelminth infectionPCRDVIefficacypolymerase chain reactionfalciparumGLURPCationic Adjuvant Formulationmalaria-exposed adult volunteersasexual blood-stage malaria vaccine...GMZ 2 adjuvantedMSPasexual blood-stage antigensCAFGMZ 2 malaria vaccine candidateanti-GMZ 2 IgG concentrationD 84STHPfSPZ Challengemalaria vaccine immunogenicity
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