PCR confirmation of TyrCopyCat in each offspring of an HDR event.

Weitzel, Alexander J.; Grunwald, Hannah A.; Weber, Ceri; Levina, Rimma; Gantz, Valentino M.; Hedrick, Stephen M.; Bier, Ethan; Cooper, Kimberly L.

doi:10.1371/journal.pbio.3001478.s006

pbio.3001478.s006.pdf (257.89 kB)

PCR confirmation of Tyr^CopyCat in each offspring of an HDR event.

journal contribution

posted on 2021-12-23, 18:48 authored by Alexander J. Weitzel, Hannah A. Grunwald, Ceri Weber, Rimma Levina, Valentino M. Gantz, Stephen M. Hedrick, Ethan Bier, Kimberly L. Cooper

(A) Schematic of primer binding locations to detect presence of the Tyr^CopyCat transgene. (B) PCR confirmed presence of the Tyr^CopyCat transgene in each of the individuals marked as positive for a gene conversion event, with families of offspring from males and females numbered as in the table in Fig 2D. Two gels were merged for ease of understanding. The raw gel images in (B) can also at the associated Zenodo data repository (https://doi.org/10.5281/zenodo.5510697) in the file labeled “S1 Raw Images.”

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