iipi_a_1942038_sm3726.pdf (277.86 kB)
Download file

Overexpression of ribophorin II is required for viability of nasopharyngeal cancer cells by regulating JAK1/STAT3 activation

Download (277.86 kB)
journal contribution
posted on 29.06.2021, 15:00 authored by Bin Wang, Weiwei Liu, Xue Jiang, Jian Li, Xiaodong Hu, Liping Li, Qianqian Gu

This study aimed to elucidate the role of ribophorin II (RPN2) in nasopharyngeal cancer (NPC) cell survival and death. RPN2 expression was upregulated in 22 human NPC specimens and 5–8F and CNE1 cells compared with that in adjacent normal tissues and normal nasopharyngeal NP69 cells.

CCK-8 and colony formation assays indicated that the silencing of RPN2 hindered the proliferation and growth of 5–8F and CNE1 cells.

RPN2 expression was upregulated in 22 human NPC specimens as well as in 5–8F and CNE1 cells compared with that in adjacent normal tissues and NP69 cells. CCK-8 and colony formation assays indicated that the silencing of RPN2 reduced the proliferation and growth of 5–8F and CNE1 cells. Annexin V/PI flow cytometry and Bcl-2/Bax analysis showed that RPN2 silencing led to increased apoptosis. Moreover, JAK1 was found to interact with RPN2, and total JAK1, STAT3, and phosphorylated STAT3 levels were dramatically decreased in cells with RPN2 silencing. Furthermore, the nuclear localization of STAT3 was blocked by the silencing of RPN2. The administration of the STAT3 activator colivelin could offset the inhibitory effect of RPN2 silencing on the survival and apoptosis of NPC cells.

RPN2 is upregulated in NPC tissues or cells, and RPN2 silencing repressed NPC cell proliferation and elicited apoptosis. RPN2 overexpression is possibly associated with JAK1/STAT3 silencing and activation. Finally, RPN2 represents a promising target for NPC treatment.

History