Optogenetic stimulation of the DRG-transplanted MGE cells enhances filtering of spikes triggered by noxious thermal and mechanical stimulation.

(A) Example of in vivo recording of the SN and DR activity (similar to these shown in Fig 1). Stimulation of hindpaw of the MGE-transplanted mice with hot water (60°C) increased firing frequency in both SN and DR branches of the nerve (onset of the bottom traces, as compared to basal activity shown in the upper traces). Application of 473-nm laser light to DRG acutely reduced heat-induced firing frequency in DR but not SN (bottom traces). (B) Summary for panel A. Two-factor (nerve site, treatment) repeated measures ANOVA: main effect associated with treatment [F(2,7) = 10.5; p < 0.05]; significant interaction between nerve site and treatment [F(2,7) = 12.8; p < 0.05]. Bonferroni post hoc test: *,**significant difference from control (p < 0.05, p < 0.01); ^##significant difference from heat (p < 0.05). (C) Similar to A and B but the hindpaw was stimulated with ice cube. (D) Summary for panel C. Two-factor (nerve site, treatment) repeated measures ANOVA: significant interaction between nerve site and treatment [F(2,7) = 47.8; p < 0.01]. Bonferroni post hoc test: *,**significant difference from control (p < 0.05, p < 0.01); ^#significant difference from ice (p < 0.01). (E) Similar to A and B but the hindpaw was stimulated with air puff. (F) Summary for panel E. Two-factor (nerve site, treatment) repeated measures ANOVA: main effect associated with treatment [F(2,7) = 11.1; p < 0.05]. Bonferroni post hoc test: *significant difference from control (p < 0.05). (G) Similar to A and B but the hindpaw was stimulated with sub-threshold von Frey filament (0.4 g). (H) Summary for panel G. Two-factor (nerve site, treatment) repeated measures ANOVA: main effects associated with treatment [F(2,7) = 32.4; p < 0.01]. Bonferroni post hoc test: *,**significant difference from control (p < 0.05, p < 0.01). (I) Similar to A and B but the hindpaw was stimulated with a needle prick. (J) Summary for panel I. Two-factor (nerve site, treatment) repeated measures ANOVA: main effect associated with treatment [F(2,7) = 235.3; p < 0.001]; significant interaction between nerve site and treatment [F(2,7) = 38.1; p < 0.01]. Bonferroni post hoc test: *,**significant difference from control (p < 0.05, p < 0.01); ^##significant difference from needle (p < 0.01). Metadata for quantifications presented in this figure can be found at https://archive.researchdata.leeds.ac.uk/1042/.

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