ppat.1009280.s009.docx (20.62 kB)
Oligonucleotides used to construct gene deletion mutants.
journal contribution
posted on 2021-08-30, 17:34 authored by TuShun R. Powers, Amanda L. Haeberle, Alexander V. Predeus, Disa L. Hammarlöf, Jennifer A. Cundiff, Zeus Saldaña-Ahuactzi, Karsten Hokamp, Jay C. D. Hinton, Leigh A. Knodler(DOCX)
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showed increased expressionremain largely unknownphysicochemical conditions encounteredpathogenicity island 1cell microscopic analysiscauses diarrheal diseaseparallel colonization strategiesepithelial cell lysisserovar typhimurium (<scv ), followedinduced expression signatures>- containing vacuoleidentified bacterial genesbacterial genes requiredsalmonella enterica bacterial releasesalmonella epithelial cellsvirulence genescytosolic colonizationzoonotic pathogenunusual lifestyletypically associatedtyphimurium withintyphimurium replicationtwo nichesuccessfully colonizestudy revealssoxs sita seq approachpermissive environmentoxidative stressmnth manganese deprivationmagnesium deprivationintracellular nichegastrointestinal tractfepb extracellular bacteriaepithelial cytosolenvironmental biosensorsentc begins withinbased rnaarchetypical vacuole
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