Observation of appressorium-mediated penetration in rice sheath cells of strains P1.2 and Δbip1 at 48 hai using confocal microscopy (63X).

Lambou, Karine; Tag, Andrew; Lassagne, Alexandre; Collemare, Jérôme; Clergeot, Pierre-Henri; Barbisan, Crystel; Perret, Philippe; Tharreau, Didier; Millazo, Joelle; Chartier, Elia; De Vries, Ronald P.; Hirsch, Judith; Morel, Jean-Benoit; Beffa, Roland; Kroj, Thomas; Thomas, Terry; Lebrun, Marc-Henri

doi:10.1371/journal.ppat.1011945.s006

ppat.1011945.s006.pdf (381.57 kB)

Observation of appressorium-mediated penetration in rice sheath cells of strains P1.2 and Δbip1 at 48 hai using confocal microscopy (63X).

journal contribution

posted on 2024-01-22, 18:29 authored by Karine Lambou, Andrew Tag, Alexandre Lassagne, Jérôme Collemare, Pierre-Henri Clergeot, Crystel Barbisan, Philippe Perret, Didier Tharreau, Joelle Millazo, Elia Chartier, Ronald P. De Vries, Judith Hirsch, Jean-Benoit Morel, Roland Beffa, Thomas Kroj, Terry Thomas, Marc-Henri Lebrun

No infection hyphae were observed in epidermal cells of rice sheaths infected with Δbip1 mutant whereas epidermal cells of rice sheaths infected with P1.2 were filled with infection hyphae resulting from penetration events. Fluorescence of WGA-Alexa488-stained fungal cells was excited with 488 nm light and is shown in green. Ap: appressorium, Co: conidium, IH: invasive hyphae, size bar = 10μm.

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