posted on 2024-03-13, 18:17authored byYan-Xia Zhang, Yi-Da Zhang, Yan-Ping Shi
Most of the traditional matrices cannot simultaneously
image multiple
lipids and phytohormones, so screening and discovery of novel matrices
stand as essential approaches for broadening the application scope
of matrix-assisted laser desorption/ionization mass spectrometry imaging
(MALDI-MSI). In this work, 12 organic small molecule compounds were
comprehensively screened and investigated as potential MALDI matrices
for simultaneous imaging analysis of various lipids and phytohormones.
In the positive ionization mode, p-nitroaniline, m-nitroaniline, and 2-aminoterephthalic acid displayed good
performance for the highly sensitive detection of lysophosphatidylcholines
(LPCs), phosphatidylcholines (PCs), and triacylglycerols (TGs). Furthermore, p-nitroaniline possessed excellent characteristics of strong
ultraviolet absorption and homogeneous cocrystallization, making it
a desirable matrix for MALDI-MSI analysis of eight plant hormones.
Compared with conventional matrices (2,5-dihydroxybenzoic acid (DHB),
α-cyano-4-hydroxycinnamic acid (CHCA), and 9-aminoacridine (9-AA),
the use of p-nitroaniline resulted in higher ionization
efficiency, superior sensitivity, and clearer imaging images in dual
polarity mode. Our research offers valuable guidance and new ideas
for future endeavors in matrix screening.