Three new cyclopentenoneacrylic acid derivatives, trichodermacid A (1), trichodermester A (2), and trichodermester B (3), together with thirteen known compounds, were isolated from an ethyl acetate extract of Trichoderma atroviride H548, a fungus derived from mangrove sediment. The structures of the new compounds were elucidated by spectroscopic methods including HR ESI-MS, 1H NMR, 13C NMR, and 2D-NMR techniques. The antifungal activity of the isolated compounds was evaluated against tea pathogenic fungus Pestalotiopsis theae. Trichodermester A (2) showed potent anti P. theae activity with MIC of 125 μg/disc, while the other compounds were inactive.
Funding
The project was supported by COMRA Project of China (DY135-B2-16), National Basic Research Program of China (973 Program) (No. 2015CB755901), the Xiamen Ocean Economic Innovation and Development Demonstration Project (16PZP001SF16), Fujian Key Science and Technology Program (No. 2018N0017), Scientific Research Foundation of Third Institute of Oceanography, SOA. (No. 2017002) and Xiamen Science and Technology Program (No. 3502Z20182029).