posted on 2023-11-15, 16:37authored byJiarong Guo, Yulin Zhu, Peng Miao
Nano-impact electrochemistry (NIE)
enables simple, rapid, and high-throughput
biocoupling and biomolecular recognition. However, the low effective
collision frequency limits the sensitivity. In this study, we propose
a novel NIE sensing strategy amplified by the CRISPR-responsive DNA
hydrogel and cascade DNA assembly. By controlling the phase transition
of DNA hydrogel and the self-electrolysis of silver nanoparticles,
we can obtain significant electrochemical responses. The whole process
includes target miRNA-induced strand displacement amplification, catalytic
hairpin assembly, and CRISPR/Cas trans-cutting. Thus, ultrahigh sensitivity
is promised. This NIE biosensing strategy achieves a limit of detection
as low as 4.21 aM for miR-141 and demonstrates a high specificity
for practical applications. It may have wide applicability in nucleic
acid sensing and shows great potential in disease diagnosis.