Multimetal-Based
Metal–Organic Framework System
for the Sensitive Detection of Heart-Type Fatty Acid Binding Protein
in Electrochemiluminescence Immunoassay
posted on 2024-02-29, 06:43authored byYuan Li, Guanhui Zhao, Bing An, Kun Xu, Dan Wu, Xiang Ren, Hongmin Ma, Xuejing Liu, Rui Feng, Qin Wei
In
this work, an electrochemiluminescence (ECL) quenching system
using multimetal–organic frameworks (MMOFs) was proposed for
the sensitive and specific detection of heart-type fatty acid-binding
protein (H-FABP), a marker of acute myocardial infarction (AMI). Bimetallic
MOFs containing Ru and Mn as metal centers were synthesized via a
one-step hydrothermal method, yielding RuMn MOFs as the ECL emitter.
The RuMn MOFs not only possessed the strong ECL performance of Ru(bpy)32+ but also maintained high porosity and original
metal active sites characteristic of MOFs. Moreover, under the synergistic
effect of MOFs and Ru(bpy)32+, RuMn MOFs have
more efficient and stable ECL emission. The trimetal-based MOF (FePtRh
MOF) was used as the ECL quencher because of the electron transfer
between FePtRh MOFs and RuMn MOFs. In addition, active intramolecular
electron transfer from Pt to Fe or Rh atoms also occurred in FePtRh
MOFs, which could promote intermolecular electron transfer and improve
electron transfer efficiency to enhance the quenching efficiency.
The proposed ECL immunosensor demonstrated a wide dynamic range and
a low detection limit of 0.01–100 ng mL–1 and 6.8 pg mL–1, respectively, under optimal conditions.
The ECL quenching system also presented good specificity, stability,
and reproducibility. Therefore, an alternative method for H-FABP detection
in clinical diagnosis was provided by this study, highlighting the
potential of MMOFs in advancing ECL technology.