Multicolor Chemiluminescent Resonance Energy-Transfer System for In Vivo High-Contrast and Targeted Imaging

Chemiluminescence (CL) resonance energy transfer (CRET) has received great attention due to its fascinating applications in in vivo imaging and photodynamic therapy. Here, we report a highly efficient CRET polymer dot (CRET-Pdots)-based system using catalytic CL reagents as energy donors and fluorescent polymers and dyes as energy acceptors. CRET-Pdots consist of Fe­(III) deuteroporphyrin IX (CL catalyst), fluorescent polymers, and dyes. The CL intensity and duration are markedly enhanced by using ultrasensitive catalytic CL reaction of luminol analogue-H₂O₂, and the CL emission wavelength can be adjusted by one-step/two-step energy-transfer strategies. CRET-Pdots show intensive multicolor CL (about 3000× enhanced), an adjustable emission wavelength (470–720 nm), long CL duration (over 8 h), and a high CRET efficiency (50%). CRET-Pdots possess excellent biocompatibility, sensitive response to reactive oxygen species (ROS), and ultrahigh catalytic activity. They are successfully used for high-contrast real-time ROS imaging and in vivo tumor-targeted imaging with an excellent signal-to-noise ratio (over 90).