posted on 2022-12-19, 13:03authored byYawei Feng, Yunshan Yang, Yating Xiao, Ting Fu, Lei He, Lubin Qi, Qiuxia Yang, Ruizi Peng, Weihong Tan
Extracellular vesicles (EVs) have emerged as a potential
biomarker
in liquid biopsy. However, cancer heterogeneity poses significant
challenge to precise molecular diagnosis based on single-parameter
input. Hence, strategies for analyzing multiple inputs with molecular
computing were developed with the aim of improving diagnostic accuracy
in liquid biopsy. In the present study, based on the surface of aptamer-encoded
EVs, three toe-hold extended DNA aptamers served as specific inputs
to perform AND-logic-gating to distinguish between healthy and cancerous
EVs. In addition, this strategy has been successfully employed to
analyze circulating EVs in clinical samples from colorectal cancer
patients and healthy donors. The developed method has a promising
future in the analysis of multiplex EV membrane proteins and the identification
of early cancer.