pgen.1010882.s002.pdf (10.54 MB)
Motility assay example pictures.
journal contribution
posted on 2023-11-27, 19:11 authored by Joel Hallgren, Kira Koonce, Michele Felletti, Julien Mortier, Eloisa Turco, Kristina Jonas(A) Representative motility zone (soft agar colony) pictures in grayscale and the spectrum color lookup table of ImageJ (ver 2.1.0) after 72 hours incubation. All pictures are in scale (scale bar: 10 mm). (B) Micrographs of cells withdrawn from motility agar colony edges after 72 hours incubation, exhibiting clear phosphate starvation phenotypes in M5G prepared without phosphate. Scale bars: 5 μm.
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upon nutrient depletionstarved swarmer cellsstarved stalked cellssessile stalked cellsmotile swarmer stagemotile swarmer cellsdna replication initiationdna replication controldevelopmental program eithercommon mechanism involvingbacteria stop proliferatingsessile stalked cellreplication initiator dnaamotile swarmer celllimited dnaa availabilityarrest cell developmentthree starvation conditionsdistinct starvation conditionscertain starvation conditionsdecouples cell differentiationdiv >< pc ), nitrogencaulobacter crescentus starvation conditionscell differentiationcrescentus dnaa translationcell cyclec n starvationundergo physiologicalsignaling moleculesprevents ppoorly understoodmorphological changesmain reasonfindings demonstratedimorphic bacteriumdata suggestconsequently absencecellular responses
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