posted on 2021-03-23, 14:37authored byQiang Zhang, Zehua Guo, Fang Luo, Hua Xiao, Weiwen Liu, Liuyin Fan, Chengxi Cao
Herein,
the quench model of the moving exchange boundary (MEB)
was first created via a ligand of 5,5′-dithiobis(2-nitro-benzoic
acid) (DTNB) and group of 3-mercaptopropionic acid (MPA) capped on
QDs, and then the recovery model was formed via MPA and 2-nitro-5-thiobenzoic
acid (TNB) capped on QDs. The theory on MEB dynamics and width was
developed based on the two reversible models, the simulation was conducted
for the illumination of MEB, and the protocol was described for the
MEB runs. The experiments revealed that (i) the quench model could
be created via DTNB and MPA capped on QDs and the recovery one could
be in situ formed via MPA and TNB capped on QDs,
showing the feasibility of MEB models; (ii) the simulations on MEB
dynamics and width were in coincidence with the theoretic predictions,
showing the validity of two models; and (iii) the experiments demonstrated
the validity of models, predictions, and simulations. The models and
theory have potential for development of a biosensor, nanoparticle
characterization, separation science, and an affinity assay of ligand-QDs.