pbio.3002544.s012.pdf (287.89 kB)
Microscale thermophoresis (MST) titration.
journal contribution
posted on 2024-02-29, 18:53 authored by Carlos A. Rodríguez-Salazar, Sarah van Tol, Olivier Mailhot, Maria Gonzalez-Orozco, Gabriel T. Galdino, Abbey N. Warren, Natalia Teruel, Padmanava Behera, Kazi Sabrina Afreen, Lihong Zhang, Terry L. Juelich, Jennifer K. Smith, María Inés Zylber, Alexander N. Freiberg, Rafael J. Najmanovich, Maria I. Giraldo, Ricardo Rajsbaum(A) Microscale thermophoresis (MST) titration curve for the VP35 Ubiquitin interaction. Normalized fluorescence (Fnorm) for Tagged WT VP35 in complex with different concentrations of Ubiquitin were measured by MST. The estimated Kd is 15 nM. (B) Microscale thermophoresis (MST) titration curve for the VP35 SFC interaction. Normalized fluorescence (Fnorm) for Tagged WT VP35 in complex with different concentrations of SFC were measured by MST. The estimated Kd is 375 nM. The data underlying the graphs shown in the figure can be found in S1 Data.
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